The procedure of yeast proteome analysis using protein chip technology. 

The yeast ORFs were cloned into a double tagged yeast GAL1 expression vector 
via a recombination strategy and verified for correct identities by sequencing.  
Each pure plasmid construct was then reintroduced into a yeast strain for large-
scale protein purification.  Yeast cultures were grown in a 96-well format and 
induced by addition of galactose.  After the high-throughput purification step, 
the purified proteins were aliquoted and stored in a glycerol buffer at ¨C80¡ãC 
before printing.  Using a high precision microarrayer, 6566 protein samples can 
be double-spotted onto 80 slides in a single experiment.