Global Analysis of Protein Activities Using Proteome Chips

On-line supplement to Zhu, H. et al. (2001) [PDF]

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Figures from the paper Table of phospholipid and calmodulin binding proteins detected by the proteome microarrays Procedure of yeast proteome analysis using protein chip technology Immunoblot analysis of proteins purified from 3 ml yeast cultures A proteome chip probed with anti-GST antibodies Distribution of errors between duplicated spots of each ORF Examples of different assays on the proteome chips A putative calmodulin-binding motif Analysis of the phosphotidylinositol lipid-binding proteins strong and specific strong and nonspecific weak and specific weak and nonspecific Confirming protein-lipid interactions detected by the proteome microarrays Supplementary Dataset Normalized green signal of all the ORFs in phospholipid binding experiments List of ORFs found positive in phospholipid binding experiments Explanatory slides [JPEGS] [PPT] Protocols and Algorithm Design Protocol for protein expression and purification Algorithm for identifying positive signal on proteome chips Download entire dataset of   every ORF on the chip phospholipid-binding proteins only raw signal -- signal and flag of twin spots in GST and phospholipid binding experiments normalized green signal -- normalized signal (mean of twin spots) in phospholipid binding experiments. -10000 indicates spots that are flagged as bad green vs red ratio -- ratio of phospholipid binding to GST binding signals, including 90% confidence interval

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H Zhu, J Klemic, S Chang, P Bertone, A Casamayor, K Klemic, D Smith, M Gerstein, M Reed, & M Snyder (2000). Analysis of yeast protein kinases using protein chips. Nature Genetics 26: 283-289. [WebSite] [PDF]

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