Tues Sept 8th      Molecular interactions in biology. Ligand binding and multiple equilibria, Scatchard analysis
LR

Thurs Sept 10th  Cooperativity and allostery as regulatory mechanisms
LR

Tues Sept 15th    Basic spectroscopic methods
LR

Thurs Sept 17th  Protein packing and hydrophobicity (i) Experimental
LR

Tues Sept 22nd   Protein secondary structure (i) Experimental
LR

Thurs Sept 24th  Protein secondary structure (ii) Statistics and prediction
MG

Tues Sept 29th    Protein packing and hydrophobicity (ii) Theory and  geometry
MG

Thurs Oct 1st      The equilibrium stability of macromolecules
LR

Tues Oct 6th       Practical demonstrations
LR

Thurs Oct 8th     Separation methods. How do we purify macromolecules  to study? Different requirements for different techniques of study. Partition coefficients and
LR                       chromatographic methods.
 

Tues Oct 13th    The basic forces and interactions that stabilize macromolecules
MG
 
Thurs Oct 15th   Detailed electrostatics
MG

Tues Oct 20th    Molecular shapes and surfaces
DME

Thurs Oct 22nd ****MIDTERM****

Tues Oct 27th    Size and shape-based separations: ultracentrifugation, SDS electrophoresis and more.
DME

Thurs Oct 29th   Solution scattering methods: light, x-rays, neutrons
DME

Tues Nov 3rd     Newly emerging techniques: Biacore and mass spectroscopy
DME

Thurs Nov 5th    EGF-receptor interactions: a showcase for combining calorimetry and SAXS
DME 

Tues Nov 10th   Practical demonstrations
LR

Thurs Nov 12th  Simulation
MG

Tues Nov 17th   Water
MG

Thurs Nov 19th  Practical demonstrations
LR

Tues Nov 24th ****Thanksgiving Break****
Thurs Nov 26th

Tues Dec 1st      Protein folding. What do we know and how do we know it? Pathways, rate determining steps, current models and controversies.
LR

Thurs Dec 3rd    Protein miss-folding and association in vitro and in disease.
LR 

Reading period begins December 7th. The date of the final exam will be announced during the semester.