MBB 400a/700a
Problem Set #1


1. Assume that the dimensions of a single E. coli cell are scaled up to the size of East Rock (the big natural landmark you can see from looking north out the window of Bass).  You may estimate the height=300 feet and the diameter=600 feet.  What would be the size (diameter) of the following cellular components?  What other objects are of a similar size (i.e. a baseball)?
Ribosome
tRNA molecule
Water molecule
Small protein (20 kD)
Large protein (200 kD)

Now assume that a Eukaryotic cell is the size of East Rock.  What is the thickness of the cell membrane?  What is the relative size of an E. coli cell?

2. You have a 20 residue polypeptide in an a-helix conformation and a 20 residue polypeptide in a b-sheet.  Which is longer and what are the approximate lengths of each?

3.  Estimate how many small proteins can fit inside a ribosome.  You may assume both are spherical and estimate the diameters.  Show your work.