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Analysis of Yeast Protein Kinases Using Protein Chips


For the autophosphorylation assay, the kinases were directly adhered to the treated PDMS wells and 33P-g-ATP was added; for substrate reactions, the substrates were bound to the wells, and then kinases and 33P-g-ATP were added. After the reactions were completed, the slides were washed and the phosphorylation signals were acquired and quantified using a high resolution phosphoimager. To identify kinase activities, the quantified signals were converted into fold increases relative to GST controls.

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Protein Kinase Phosphorylation Data (log fold increases)
KinaseAutoAxl2CaseinpolyEYGic2HistoneHog1MBPMek1Rad9Red1Swi6Hsl1Tub4Ptk2Hog1DGST
YAL017W1.2327907651.4317160611.4843888581.2179125661.234997561.0905891481.8462465951.5327998641.7307611610.8236885011.5080214362.3893397231.1214715761.6048791521.8469243131.8455688780.823763504
YAR018C0.7150809010.5441047730.5265365360.8301846420.4257565930.2663903161.1985650150.7481695521.0124397620.87228950.4183488850.6275415560.5834074240.8934344441.1986088841.1985211460.936122186
YAR019C0.6545043060.8430382560.7885470830.8941899451.0048886050.5978671121.1448446771.231694290.9757674381.0402239181.0944836592.2907687980.699995381.1169105861.1472045991.1424847550.612029322
YBL016W0.8337420920.7848214320.8459452821.3921585661.745358460.9809608111.0748833381.1437685350.9041121512.5330509191.3233640971.4645186270.5622716841.4644531511.0689097081.0808569680.898443189
YBL105C1.2107007190.4989277220.5952481591.464338070.568782880.4864361530.8001681060.731872930.6731456220.6609246440.5936893520.6987063890.2426732630.8130141090.7836187370.8167174740.021164371
YBR028C0.4564961810.5645186160.4133691380.7518561921.6529523370.3627902810.8856392410.8989970411.0946714152.3850813920.5874486291.1310175520.4716344150.8675501970.8723197090.8989587730.040164604
YBR059C0.5776596380.5517332341.1890264280.5584374750.6423192870.546401770.9872609590.7645573430.4171859921.0194972860.6361985340.6165483780.6230971020.8626235030.9806965130.9938254040.026928042
YBR079W0.1557642450.6448144230.6094651990.5712570170.5622523730.3009704161.2300406121.1978337050.6567719492.1012914121.7482150470.5634441460.687910520.8216409851.2410053921.2190758320.08513697
YBR136W0.4013472992.6142261681.280390640.2879200660.200475950.2954572891.209923210.5376928740.7037435660.6803781520.429948850.3006912250.6207249610.335215231.2169783661.2028680550.12042263
YBR160W0.3288819360.7834585320.6859964920.4811371120.5037780340.620095981.0385732290.8013119710.6131859660.6660178620.9656077180.621509560.7233872740.9973081941.0404772781.0366691790.058657125
YBR274W1.0042595480.379099670.3857036350.388528750.7236467910.5597304470.9716749220.3814801780.6105973880.6450448850.4072619120.5422539210.271658610.6026987450.9776649530.965684890.020883025
YCL024W0.3980854990.651927250.7470725030.5386540890.6207888761.6469439890.9745629830.8550502180.577482170.5573441710.7230605630.8359072070.3921607680.856709270.9861401170.9629858490.24429382
YCR008W0.2320876110.306541820.4468898030.3115089430.7451217620.4145459340.669727690.5444986270.7768417480.5906699950.354341480.5915051110.3751002582.1113350880.6828169680.6566384130.331179525
YCR073C0.8230372490.3412906410.7331984530.1879645250.2347120740.1534464831.0122580120.4027619820.8127862561.5832178541.520254050.5367873670.161862650.3561695451.0071110571.0174049680.011151922
YCR091W0.318349270.2252576930.7501346010.4082765330.2403859240.0170730490.9274500730.7056020670.4280229690.7240402650.5209979850.5371798460.9825019730.6826376210.9199839010.9349162440.09209971
YDL017W0.538101040.4746888940.4128133870.7452284270.6717672010.3039186591.4943928141.0121763990.6410760021.3081807850.3636173950.7598549570.6502392351.0235102641.5061453021.4826403270.008235673
YDL025C0.7463785670.838160070.7889109990.9126437410.6446227490.6008703081.6403509940.9834670571.0343229280.8572956010.9348795250.7386655130.8063280141.0872823521.6459156141.6347863730.311490124
YDL028C1.3823194882.5764835092.3127426842.2208962582.3782410162.0226953962.2583035972.0172665851.9965270682.1316936912.5086646852.1828683782.0358426822.3494232962.4889516632.0276555320.893893365
YDL079C1.5530289860.7412350540.7637790990.5865122630.6969346820.3093281061.1188867981.5627072170.6050016150.8267583270.7235834241.6314392480.5204269160.8060185641.1228499641.1149236320.229751261
YDL101C1.9187279542.2439732081.9016144033.1829806541.4096823641.6950533322.1181482191.7827626611.8381926161.7329883822.0503265221.7271486381.8190482731.97360082.2086409062.0276555321.228463252
YDL108W0.9117408040.7406012541.203665521.0742739641.5009662720.7125030212.269065371.069652641.4986878622.5269673771.1068985191.0661435340.8212442881.2067288562.5104752092.0276555320.515552305
YDL159W0.6450342040.4643962210.8375729380.9197719151.9423284660.3612584710.9534379170.7852691290.7759603031.5887690510.7666639060.8795693960.5142431650.9459023580.9232262240.9836496110.814168308
YDL214C0.7085015060.8236118920.8997884550.7034482730.7752551740.5002714531.1110887840.7562115561.1514003450.9213512340.9466288960.7404537040.4409215480.6483076441.1006155051.1215620620.038758935
YDR052C0.5526000630.4555369210.7036986070.5656871440.4151431470.403397591.2272645820.7192489270.6010488011.0203626930.6646017520.6514935510.5329141930.7683330831.2373784281.2171507371.051785193
YDR122W1.2216648871.0863187550.716583830.9784540430.8013975052.6142867671.1606529620.9590656651.875116431.0845024660.8319574581.6586677250.7148315890.8654493991.1588724061.1624335170.396676131
YDR247W 1.0881632411.1232929752.1160895961.1691622991.3816176991.7901682651.1653085871.0709017011.2099628951.6352790011.3579109652.6233743280.5235160411.3406163351.1800413661.1505758080.978508657
YDR466W0.603286870.3165023631.0084545861.2079773770.8669762321.0688792340.5757862470.9344087861.413482921.0341865771.4063610431.155186170.2873938241.2112438450.5718482270.5797242670.164724628
YDR477W0.3028865110.284734220.3663324690.3712217750.3143398440.434261120.7905931980.5245101170.4076640770.5952535630.2954372960.4874688110.3994840760.4169887960.7944564440.7867299520.283552712
YDR490C0.8899319540.4690378340.6467441050.634808850.4779960710.5390451351.1095773781.0596224740.6309985581.4491315620.6636641380.5093711060.5162502120.8368107731.0929603921.1261943650.09583111
YDR507C1.2267032570.8714939391.4260247331.4012779931.0726545711.4937141391.9971108721.5284284181.2980643621.4631855321.2520168132.1969966281.2948438421.6311845712.0001194161.9941023280.860546738
YDR523C0.5014811810.8189951170.5783484930.7225540740.4800936640.4087209481.2487964630.7748186440.8933131211.4829219250.6549020780.8911966741.209488830.7648675781.2394511391.2581417870.1106863
YER123W1.7603044781.4495335551.6118712481.7805361341.8632135551.7117307422.1870614641.8720048031.7715522282.7787199241.8717124151.6190674151.7903888121.9665491612.3464673972.0276555320.659582412
YER129W0.676229091.7808209210.6935345410.7310297970.6887484110.6353842211.3293382760.9448384631.6892323730.7988330470.8098711531.2134427020.8224565491.0176904751.323471931.3352046220.346993726
YFL029C2.0036068811.4246668040.9048307420.8877132040.7321799710.8745057181.4622846170.8922872180.8957828950.987045720.8346060650.9141256590.7050910041.0418195791.454952181.4696170550.355851991
YFR014C2.8921955232.5358040512.650241582.432927862.9250415112.8881018952.3086676532.2520212633.0613155832.6510962263.1019284042.8298716492.1250620053.4045244062.5896797752.0276555320.894176982
YGL059W1.3660814320.7676048581.0072328291.0836165540.8458034870.5473371591.2728419730.8925123320.9966928881.4481472770.9791285311.2343266510.8910792550.9746553891.2642258441.2814581010.458344154
YGL158W0.6606755180.8710140131.7887568340.8589273750.7504729420.6089482461.0402719631.4582197291.1487312822.3324714820.8639947810.8665406580.7137385610.8465396341.0280434351.0525004910.204677804
YGL179C0.654528380.5001128630.9973551610.6431816370.6637281540.5989497590.9315088340.7616361610.7701606371.0984421291.5561555740.7776536760.937414890.6608506270.9178565690.9451610990.131057612
YGL180W0.6761792850.8106985340.7198917830.795426520.7301177280.6719977071.5367313970.9028903570.9260504210.8069352430.698804640.7305590080.6204311690.7251388761.5460082071.5274545870.180044226
YGR040W0.2268507050.3056610880.4160696080.8292376870.5323870720.5400408730.8269206840.6249503480.6955147030.7936999750.45085770.6087683060.577011270.530796740.825478370.8283629990.066961851
YGR052W0.3352276840.5067852870.5165848190.5665875220.5562397950.5759977231.4922334430.6154235290.5564091670.7846111390.5202996340.4443227270.8526106420.4884222881.5043750631.4800918240.060015473
YGR080W0.2335970420.1304854840.7908414260.5279669530.2908632950.2818894620.7295629390.5456527060.5351447370.734220790.4763637790.4872345530.4416088691.2735430460.7243203410.7348055371.022175996
YGR092W0.9929041420.7729613960.8420953140.4806071830.391121950.3684359051.1113098870.9118447220.6572110450.8989512110.6492070480.6661903920.6071621240.831928761.1176359561.1049838180.368303413
YGR188C0.3855167640.6948758390.5974498422.5690086150.8463741260.3708992451.1272395211.0107818630.8246811920.7753702240.6708991940.4545823830.6161753370.6647984381.1258321091.1286469330.043457985
YGR262C 0.5239134160.7030492610.8032352650.5815201040.5097806930.4520162451.1250428260.6970323690.8428770660.7632493890.6597041360.5459714410.9782867730.546622811.1207205641.1293650880.142225159
YHL007C2.2614025142.5524568292.0377309781.9233454531.9849155932.1593446472.3086676532.1484557742.0551029832.5008732092.4895742872.2080011442.3444170652.6882014232.5896797752.0276555320.296465868
YHR030C0.651785330.8156270550.4254373630.7376284491.375882130.4916928431.2433246460.7681523750.7922593330.8508193450.6621469830.5286837781.2174596660.8026695761.2403173411.2463319520.276198896
YHR079C0.8342368351.8223996560.9518134771.0069001671.6543141151.2384335621.1518524340.7879819630.8727558552.3541950780.9024392840.7040802210.6512263230.7620805711.1487745571.154930310.959493546
YHR082C0.9969627341.1106083580.9225953451.2341695441.0261766090.97523921.2284618981.1625493532.1611971611.0498484231.2799578210.9501404521.1610579231.2429887431.2238577221.2330660740.643385674
YHR102W1.714252191.0295178470.8385132920.8674081940.7910194810.8495721181.3010700861.1712740181.1544982021.0897000361.1132293311.0518736681.0171658181.1094600131.2992998151.3028403580.911485789
YHR135C2.3562623472.4309283722.2271914662.1595931692.1099683972.0436652462.3086676531.9758232452.1604767382.2934848652.4759924432.1744494312.4914691082.5500402312.5896797752.0276555321.785690852
YHR205W1.542422610.7987552310.6260708770.6745597051.6054126650.979909791.204286360.9019195830.8495883391.0363371630.7907674140.9596674461.1269501970.8962633581.2054184731.2031542460.180046285
YIL035C0.6191905230.9704034380.800294531.4813746320.7928470390.6809794421.3501293820.9068034330.8139166020.8183728481.1969193620.7629459060.8722800451.0805807681.3538999411.3463588220.051642174
YIL042C0.6553733870.7630501590.5362197281.3654686170.8682893570.8313823151.1388071540.6777563790.8365249780.8381582090.8505294751.1914886480.8900919230.6177034791.1430616361.1345526710.019968551
YIL095W0.8422170310.7290724170.6474922890.650438220.8003280570.3356027741.0486688880.569623640.9093232352.0590759340.6529977860.7102390490.7068966290.6221487861.0471687311.0501690450.043196583
YIL147C1.2586463420.6743327440.7253545940.3372757650.4082398730.3626852020.9485645970.6042596180.6288138870.5790937210.5639336750.5526364840.6539040970.5644357140.94708940.9500397930.059636105
YJL006C0.629495220.4185465960.541947481.0925745770.3204550070.5770738071.0317916950.8087120760.8569953860.5115688130.5535715650.6700164110.6425926982.4944747941.0296083651.0339750250.015281604
YJL057C0.2720162780.1429442020.414853680.5884237711.04958270.0729744661.5071293880.6681599220.9575725540.6092005750.6245915020.7310994040.3591561980.5346458651.5175804551.4966783220.020569149
YJL095W1.2168751970.5404367420.9987377051.0091215081.6431739060.7927618371.7960061830.8717594951.104721550.932993091.0927625130.6463082730.538287311.1544344771.7993917051.7926206610.705205928
YJL106W1.0559409681.054409091.0486815461.2324560421.0804188711.1159527771.5337834581.4787490681.257899121.1086214011.3034231761.0435502631.46113571.5210257721.5362375591.5313293570.520566965
YJL128C0.5040952940.6930983590.5459033392.1924894030.6917913630.3345766910.8693317650.647493471.3483563120.7969713690.4657424781.3597093130.722824590.5969285990.8664962270.8721673021.267710992
YJL141C1.6633457322.4152971761.7374582591.6810404581.1563196381.6247977092.1730295391.8275340621.8282853591.8413323122.0624820631.8241880021.9049886551.7965037042.3184035462.0276555320.891761207
YJL164C1.7763655781.8036878040.8686261540.7561201710.4790209130.5977057661.2423667531.3593743811.5814238790.9408365711.3504215940.7644671510.8080427840.7104636661.2448976571.2398358490.021795931
YJL165C1.5084713820.7165537390.5771531340.5386811340.9892563370.4014485740.8608680850.7048525271.0121076251.2708349450.4680826880.4920031880.5751145350.4634586370.8562317980.8655043720.02571287
YJL187C0.8752371091.2093014180.8778056971.3874471710.6156796690.8215194821.4964684732.0976608170.9823291860.8455512240.931609220.7194758181.0182720130.7614178331.4988051011.4941318440.239300889
YJR059W0.4903475292.1451976360.5099868780.9740213330.9010087310.3363654391.9390173930.6952326860.8075813480.7752129510.7389401180.2521835541.5818924570.6749293061.9482764591.9297583280.090139478
YKL048C0.3703010420.3944900830.9834180020.6205888440.6011475590.1078266521.3054336030.6587427520.6038331070.8139268030.4500376060.2904651170.5418408180.5790205651.3183368821.2925303240.023698601
YKL101W0.4146940190.7848426850.4437456080.6791869350.7584332960.5160146551.0530192190.733835030.6281497490.6500718180.6103808450.6402241420.7488688661.96877371.0580717321.0479667060.079096063
YKL116C0.1697862581.4423523990.3260184350.3398510430.3554448220.7733150150.90844660.7630989721.1710995722.1134665330.4076967910.5790745960.5681096830.3635721740.9125810390.904312160.027125274
YKL126W0.5272378820.5600883790.4721878910.4801061460.4128814430.524971340.7110213350.8734271240.5228473460.8356529440.5791569440.5206448490.5580833670.8343646380.7133777730.7086648980.060132908
YKL139W0.3620247530.4330730450.6673557361.9730250270.3191001560.2390031921.2416237350.6598780470.5290819930.6861802660.6509648940.5788950330.5109645171.0279003871.2517585191.2314889510.214653493
YKL161C0.5027072550.5662578561.5318094530.6440296270.2963708120.4921954641.159231030.9078825180.6461217761.298458290.8385928110.5862320410.7900997540.7104244111.1618963871.1565656730.209367299
YKL166C0.3574505910.5521797140.5053935780.4704362310.462672511.6687339571.1083989330.6780328710.5483696770.9353690412.2751364260.5282597560.4556941550.8631036771.1109039611.1058939050.088749624
YKL168C 1.4053985150.4901953290.4828097160.5353666110.7598839160.5851268421.3942768490.9812022161.4480024831.4003492630.5473250070.6286486420.8079941010.5622652571.3781273611.4104263380.009673061
YKL171W1.3446832081.2057379121.530610461.3274107611.5504562891.484949621.6160818351.5271232141.6089784351.6041568071.6462794651.5865815061.2038660412.0940900041.6259805921.6061830780.869225572
YKL198C0.873557610.6770727690.9749332961.1517524260.8592056681.3726526691.2558517171.5123450951.2960599441.0547901221.1237207321.1463181950.8634389651.5900389591.2605707991.2511326340.957713495
YLL019C1.2921668991.4763543671.5827974261.6418704981.3347589811.9095147361.6989707341.8196864971.5766412341.8782659622.0237262831.8856638271.4906344772.1112390181.6995525681.69838891.036207748
YLR096W1.8306856172.4799502081.6601701291.459134911.422230941.562621251.8937115891.7338857641.6297661791.1243035261.8735028281.5305726081.5612896451.72422181.8955564651.8918667141.104373259
YLR113W0.3290953230.899833010.5463746410.6161031420.2905636510.2828515960.995792140.6467773160.683290930.5924687630.9739375710.4931839830.3964672610.5500970560.9958892470.9956950330.026305179
YLR248W1.1151720161.0586050471.2554997051.2859672991.3677012841.3021801011.4914587011.42398281.2552011441.1265683341.3423116621.421479411.0790982872.3308013841.4962839491.4866334520.720890389
YLR362W0.248310220.5506756482.2792532550.3645038860.4471819090.0558679861.0699229460.7854540970.6445885890.4192551750.6271879251.0797194450.4987620570.4127659491.0716113861.0682345060.004715309
YML112W0.2854590540.4110967370.3694208012.3871004590.3060587450.4280532871.2417038280.7861006360.5430450840.3276599120.4137772610.6373393830.7166526030.6608602531.2431822351.2402254210.032551149
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YPL026C1.2125361840.7062832431.8029268191.0965031950.9959092691.1675165251.2667966151.3347922091.4313016871.4817606271.5282517171.1124150981.0814902771.8111758661.2765521651.2570410660.17513313
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YPR161C0.1904418250.4253703840.431708380.3103878650.4666240720.3583800650.5005883740.4833436140.4145539770.8345430920.4447139560.2727046020.1162621170.592817980.4959354940.5052412540.001448305
YOR351C0.7475534441.2532897050.7236151910.7123268190.8503594060.6364448841.1384596781.4539142810.9001630.7427126390.9895846521.8966727640.8318304990.8824553641.1457808771.1311384780.18119552



Paul Bertone
6/15/00