#!/usr/bin/perl print "Content-type: text/html\n\n"; print (""); print ("
For the autophosphorylation assay, the kinases were directly
adhered to the treated PDMS wells and 33P-g-ATP was added; for substrate
reactions, the substrates were bound to the wells, and then kinases and
33P-g-ATP were added. After the reactions were completed, the slides were
washed and the phosphorylation signals were acquired and quantified using
a high resolution phosphoimager. To identify kinase activities, the
quantified signals were converted into fold increases relative to GST
controls.
");
print("
Download Table"); print("
");
$local_data = "kinase.dat";
print ("");
print (" ");
print ("Protein Kinase Phosphorylation Data (log fold increases) \n");
open (DATAFILE, "<$local_data");
while (Kinase ");
print ("Auto ");
print ("Axl2 ");
print ("Casein ");
print ("polyEY ");
print ("Gic2 ");
print ("Histone ");
print ("Hog1 ");
print ("MBP ");
print ("Mek1 ");
print ("Rad9 ");
print ("Red1 ");
print ("Swi6 ");
print ("Hsl1 ");
print ("Tub4 ");
print ("Ptk2 ");
print ("Hog1D ");
print ("GST ");
print (" \n");
}
print ("$orf ");
print ("$Auto ");
print ("$Axl2 ");
print ("$Casein ");
print ("$polyEY ");
print ("$Gic2 ");
print ("$Histone ");
print ("$Hog1 ");
print ("$MBP ");
print ("$Mek1 ");
print ("$Rad9 ");
print ("$Red1 ");
print ("$Swi6 ");
print ("$Hsl1 ");
print ("$Tub4 ");
print ("$Ptk2 ");
print ("$Hog1D ");
print ("$GST ");
print ("
");
print ("Paul Bertone
6/15/00
");
print ("\n");
exit(0);