Genomic and proteomic analysis of the myeloid differentiation program

A mouse promyelocytic cell line (MPRO), derived from murine bone marrow cells and arrested developmentally by a dominant-negative retinoic acid receptor, morphologically differentiates to mature neutrophils in the presence of 10 mM retinoic acid (RA). We have prepared an extensive catalog of the gene expression changes that occur during morphologic maturation. To do this, we used 3’-end differential display, oligonucleotide chip array hybridization, and two-dimensional protein electrophoresis (2DE). We identified a large number of genes whose mRNA levels are modulated during differentiation of MPRO cells.

We used an oligonucleotide chip containing 13,179 probe sets corresponding to about 7000 murine genes to analyze patterns of mRNA expression in the same RNA samples used for DD. The information obtained by oligonucleotide arrays was collected in the database dbMCa. The gene information detected by DD was collected in database dbMCd.

1. To access “dbMCa”, you can click AffyData037_MPRO database. In “AffyData037_MPRO”, go to “form”, then click “F411_M02_Mu13Ksetw/sF31” you will access a gene card with all the information for it. This is a large database to download.
2. To access “dbMCd”, you can click YaleDB_ZL034 database. In “YaleDB_ZL034”, go to “form”, then click “F04_GDT_Card01” you will access a gene card with all the information for it.