Shows an overview of the expression data sources. We looked at both mRNA
and protein abundance data from the sources listed here. In order to reduce
error rates and increase coverage of the genome, we combined and merged the
four mRNA expression datasets and the two protein abundance datasets into
two reference datasets each (the "reference expression" set for mRNA expression
and the "reference abundance" set for protein abundance). The reference
abundance set is much smaller than the reference expression set (150 versus
6071 ORFs when combined with data about the codon composition) because there
is much more data available for available for mRNA expression (from gene chips
and SAGE data) than for protein abundance (from 2D-gel experiments).
In our calculations, we only used the 4270 ORFs in the reference expression
set that have an expression level > 0.5 copies/cell since lower expression
levels are too close to the detection limit of the gene chips and therefore
too noisy.
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