To predict tertiary structure
-Use sequence analysis and low resolution spectroscopy (like circular dichroism and polarized IR) to predict a protein's secondary structural elements
-Can now use surface analysis to predict how these secondary structural elements will pack with each other
- Ridges should come into grooves in interacting faces
- Helix-helix interactions
- Based on the surfaces of alpha-helices, we may expect that they pack together only in a few different ways
- Chothia, C., Levitt, M. and Richardson, D. 1981. Helix to Helix Packing in Proteins. J. Mol. Biol. 145:215-250
- Programs for exploring and evaluating various tertiary structures, given secondary structure
- ESCHER
- Ausiello, G., Cesareni, G., and Helmer-Critterich, M. 1997. Proteins: Structure, Function, and Genetics. 28:556-567
- CHI
- Adams, P., Arkin, I., Engelman, D., Brünger, A. 1995. Nature Structural Biology. 2:154-162
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